CN104886715A - Electric shock inactivation method - Google Patents
Electric shock inactivation method Download PDFInfo
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- CN104886715A CN104886715A CN201510355720.4A CN201510355720A CN104886715A CN 104886715 A CN104886715 A CN 104886715A CN 201510355720 A CN201510355720 A CN 201510355720A CN 104886715 A CN104886715 A CN 104886715A
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- Prior art keywords
- electric shock
- ablation method
- plate
- conducting medium
- power supply
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/32—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with electric currents without heating effect
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/85—Food storage or conservation, e.g. cooling or drying
Abstract
The invention discloses an electric shock inactivation method. Electric shock inactivation equipment is employed while a low-voltage pulse source or a normal alternating-current power source acts on a conducting medium to generate current penetrating the conducting medium so as to subject microorganisms in the conducting medium to electric shock, and the microorganisms die due to losing of normal physiological functions, so that inactivation is realized. The electric shock inactivation method has the advantages of avoidance of voltage increase, heating and high-voltage power sources, simple equipment structure, low energy consumption, safety and pollution avoidance.
Description
Technical field
The present invention relates to inactivation technology field, be specifically related to a kind of electric shock ablation method.
Background technology
Microorganism is almost present in all fields natural, and naked eyes generally be can't see, and needs to use microscope just can observe.Food is placed at normal temperatures, will soon be subject to microbial contamination and invasion and attack, causes food spoilage and goes bad.The microorganism of food apoilage is caused to have bacterium, saccharomycete and mould etc., they can produce various enzyme material in Growth and reproduction process, destroy cell membrane and enter cell interior, the nutriment in food is decomposed, food quality reduces, and then makes food occur rotten and rot.Edible food that is rotten and that rot can cause a lot of disease.So, it may be necessary and the period of storage that inactivation treatment carrys out food and extend is carried out to food, prevent by the generation of the diseases induced thing of microorganism.
High-temperature inactivation method is the ablation method of microorganism in modal food, general steam by heat foods to 112
oc (0.15 MPa), and maintain 20 – 30 minutes, to reach the effect of complete inactivation.The heat energy that this method consumption is a large amount of, needs pressure deactivation container, and this method can cause the rotten of nutritional labeling, also can change original taste of food.
Therefore, people are attempting multiple without heat inactivation method.
Radiological inactivation is a kind of effective ways that the electromagnetic wave utilizing electromagnetic radiation to produce kills the microorganism on most of material.Electromagnetic wave for deactivation has microwave, ultraviolet (UV), X-ray and gamma-rays etc.They can by specific mode control growth of microorganism or/kill microorganism.The patent of invention of application number 200910212077.4, discloses a kind of method of inactivating microbial in biofluid.The monochromatic light sent from one or more light source of the method effective irradiation dosage or polychromatic light irradiated biological liquid carry out inactivating microbial, the method has used a certain amount of chemical reagent, cause environmental pollution, potentially danger is existed to operating personnel, and operation is extremely complicated loaded down with trivial details.
The deactivation of food super-pressure is exactly in airtight ultrahigh pressure vessel, use water as and for medium the pressure of 400 ~ 600MPa imposed to materials such as flexible packaging foods or apply with the pressure of 100 ~ 1000MPa with high-level hydraulic pressure oil, thus kill wherein nearly all bacterium, yeast and mold.The mechanism of super-pressure deactivation is the non-covalent bond by destroying in mycoprotein, protein higher structure is destroyed, thus causes protein coagulating and enzyme deactivation.Super-pressure also can cause somatic cells film rupture, and make chemical constituent in thalline produce the various kinds of cell damages such as outflow, these combined factors effects result in microbial death.The patent of invention of application number 201110148468.1 provides a kind of continuous ablation method of superelevation isostatic cool pressing and device of liquid food, and the liquid food through squeezing the juice, filtering, clarify and allocating leaves in raw material storage tank by the method; By high-pressure pump, liquid food is continuously pumped in high-pressure bottle from raw material storage tank, reaches deactivation required pressure and after the dwell time, liquid release is also injected in intermediate storage tank.The method achieve the continuous operation of superelevation isostatic cool pressing deactivation, while reducing equipment price, improve the production capacity of equipment, but high voltage leaves very large potential safety hazard to production.
Impulse electric field (Pulsed Electric Fields, PEF) process is a kind of novel non-thermal food sterilization technology, it processes liquid, semi-solid food products with higher electric-field intensity (10-50kV/cm), shorter pulse width (0-100 μ s) and higher pulse frequency (0-2000Hz), and can form the production line of continuous sterilizing and sterile filling.The mechanism of action of current PEF to microorganism still imperfectly understands, and most scholar thinks that PEF is by external electrical field and microbial cell film direct effect, thus destroys the structure of cell membrane, forms " electroporation " and causes bacteria inactivation rate.Carry out active mass exchange between microbial cell and external environment, the integrality of cell membrane normally has extremely important effect to what ensure cell activities.The bactericidal action of PEF and it is closely related on the impact of microbial cell film.When microorganism is placed in high-pressure pulse electric, cell membrane can be destroyed, thus causes cellular content to exosmose, and causes cell death.But impulse electric field deactivation needs to use high voltage source, easily causes security incident in production, the design comparison of another electric field processing chamber is complicated, and therefore, current commercial Application is subject to a definite limitation.
Summary of the invention
For above-mentioned ablation method and Problems existing thereof, the invention provides a kind of electric shock ablation method.The method does not need pressurization and heating, and also do not need high voltage source, energy resource consumption is few, safety non-pollution.
A kind of electric shock ablation method, the method with the deactivation device that shocks by electricity for equipment, action of low-voltage pulse source or conventional AC power is utilized to act on conducting medium, form the electric current penetrating conducting medium, the microorganism be hosted by conducting medium is made to be electrically shocked effect, thus lose normal physiological function, and then dead, reach inactivating efficacy.
Above-described electric shock ablation method, described conducting medium can be the material of any conduction, as solid, liquid or gas etc., is preferably the material of flow-like, as liquid or semisolid.Best preferably liquid, as: milk, liquid glucose or liquid etc.
Above-described electric shock ablation method, described electric shock deactivation device comprises negative plate, positive plate, power supply and baffle plate; Described negative plate is connected with the negative electrode of power supply, and described positive plate is connected with the positive electrode of power supply; Described negative plate is container, is fixed on by baffle plate on container, is vertically fixed on baffle plate by positive plate with screw, leaves space between described positive plate and container, keeps suitable distance, prevents them from contacting with each other and being short-circuited.
Described power supply is action of low-voltage pulse source or conventional AC power, and its operating voltage is 5.0-30.0V.
Described positive plate is preferably made up of stainless steel or Titanium.
For splendid attire is inactivated material, described negative electrode is preferably made and can be inactivated the container of material by splendid attire, and described container is preferably made up of stainless steel.
Described baffle plate is made up of insulating materials, and described insulating materials is preferably plastics.
The invention has the beneficial effects as follows:
1. compared with needing the ablation method of high temperature, high pressure, the present invention does not need high temperature resistant or/and the equipment of high pressure, does not need heating yet, reduces the consumption of production cost and the energy, can not change by the nutritional labeling of sterilization material as food.
2. the present invention is compared with radiological inactivation, do not need use chemical reagent, safety non-pollution, does not also need high voltage source, to personnel and environment more safe and reliable.
3. electric shock deactivation device structure provided by the invention is simple, and cost is low, easy to operate, and inactivation ratio reaches 100 %, and the deactivation being specially adapted to a small amount of or a large amount of fresh milk at similar milk cattle cultivating family is fresh-keeping.
Accompanying drawing explanation
Fig. 1 is that the present invention shocks by electricity the structural representation of deactivation device.
Reference numeral: 1. negative plate; 2. positive plate; 3. power supply; 4. baffle plate; 5. screw.
Detailed description of the invention
Below with reference to specific embodiment, the present invention is further described, but do not limit the scope of the invention and range of application.
one, the making of electric shock deactivation device
the preparation method of electric shock deactivation device 1
1. the making of negative plate and installation: get high 150 mm, diameter 120mm cylindrical stainless-steel pan as negative plate (1), in the outer wall welding of stainless-steel pan, one has the electric wire link of screw hole;
2. the making of positive plate and installation: get a long 300mm, diameter 10mm stainless steel barred body as positive plate (2), plastic dam (4) is fixed on stainless-steel pan, stainless steel barred body is inserted the centre bore position of plastics plate washer (4), with screw (5), stainless steel barred body is vertically fixed in plastic dam (4), regulate the height of stainless steel barred body, make 10mm at the bottom of its lower end distance stainless-steel pan, with screw (5), stainless steel barred body is vertically fixed in plastic dam (4);
3. power supply connects: the electric wire link of stainless-steel pan is connected with the negative electrode of conventional AC power (3), and stainless steel barred body is connected with the positive electrode of conventional AC power (3), sees Fig. 1.
the preparation method of electric shock deactivation device 2
1. the making of negative plate and installation: get high 200 mm, diameter 150mm cylindrical aluminum pot as negative plate (1), in the outer wall welding of aluminum pot, one has the electric wire link of screw hole;
2. the making of positive plate and installation: get a long 400mm, diameter 20mm Titanium barred body as positive plate (2), ceramic baffle plate (4) is fixed on aluminum pot, Titanium barred body is inserted the centre bore position of ceramic plate washer (4), with screw (5), Titanium barred body is vertically fixed on ceramic baffle plate (4), regulate the height of Titanium barred body, make 15mm at the bottom of its lower end distance aluminum pot, with screw (5), Titanium barred body is vertically fixed on ceramic baffle plate (4);
3. power supply connects: the electric wire link of aluminum pot is connected with the negative electrode of action of low-voltage pulse source (3), and Titanium barred body is connected with the positive electrode of action of low-voltage pulse source (3), sees Fig. 1.
two, shock by electricity ablation method
embodiment 1
Get the electric shock deactivation device 2 made, in pot, add syrup stoste.After check circuit, power-on, pulse voltage value is 30.0V(peak value), generation current about 45.0 mA(peak value), be energized after 20 minutes, complete inactivation treatment.
embodiment 2
Get the electric shock deactivation device 1 made, in pot, add milk stoste.After check circuit, power-on, operating voltage is 8.0V, generation current about 12.0 mA, is energized after 30 minutes, completes inactivation treatment.
embodiment 3
Get the electric shock deactivation device 2 made, in pot, add milk stoste.After check circuit, power-on, pulse voltage value is 20.0V(peak value), generation current about 25.0 mA, is energized after 25 minutes, completes inactivation treatment.
embodiment 4
Get the electric shock deactivation device 1 made, in pot, add liquid.After check circuit, power-on, operating voltage is 5.0V, generation current about 8.0 mA, is energized after 50 minutes, completes inactivation treatment.
three, inactivating efficacy
Contrast with before embodiment 1,2,3,4 sample of above-mentioned electric shock deactivation device 1,2 deactivation and this sample deactivation, check bacterium, mould, Escherichia coli respectively, result shows: the present invention reaches 100 % to bacterium, mould, colibacillary inactivation ratio, refers to table 1.
Microorganism checking project result table before and after table 1 sterilizing
Claims (8)
1. an electric shock ablation method, it is characterized in that: with the deactivation device that shocks by electricity for equipment, action of low-voltage pulse source or conventional AC power is utilized to act on conducting medium, form the electric current penetrating conducting medium, the microorganism be hosted by conducting medium is made to be electrically shocked effect, thus lose normal physiological function, and then dead, reach inactivating efficacy.
2. electric shock ablation method according to claim 1, is characterized in that: described conducting medium is liquid.
3. electric shock ablation method according to claim 1, is characterized in that: described electric shock deactivation device comprises negative plate (1), positive plate (2), power supply (3) and baffle plate (4);
Described negative plate (1) is connected with the negative electrode of power supply (3), and described positive plate (2) is connected with the positive electrode of power supply (3);
Described negative plate (1) is container, is fixed on container by baffle plate (4), is vertically fixed on baffle plate (4), leaves space between described positive plate (2) and container with screw (5) by positive plate (2).
4. electric shock ablation method according to claim 3, is characterized in that: described power supply (3) is action of low-voltage pulse source or conventional AC power, and its operating voltage is 5.0-30.0V.
5. the electric shock ablation method according to claim 3 or 4, is characterized in that: described positive plate (2) is made up of stainless steel or Titanium.
6. the electric shock ablation method according to claim 3 or 4, is characterized in that: described container is made up of stainless steel.
7. the electric shock ablation method according to claim 3 or 4, is characterized in that: described baffle plate (4) is made up of insulating materials.
8. the electric shock ablation method according to claim 3 or 4, is characterized in that: described insulating materials is plastics.
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CN201510355720.4A CN104886715A (en) | 2015-06-25 | 2015-06-25 | Electric shock inactivation method |
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CN201510355720.4A CN104886715A (en) | 2015-06-25 | 2015-06-25 | Electric shock inactivation method |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN116354565A (en) * | 2023-05-31 | 2023-06-30 | 曹县人民医院 | Comprehensive treatment device for hospital sewage |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
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US6056884A (en) * | 1997-11-25 | 2000-05-02 | Japan As Represented By Director Of National Food Research Institute, Ministry Of Agriculture, Forestry And Fisheries | Continuous liquid pasteurizing apparatus and a method therefor |
CN1623447A (en) * | 2004-12-09 | 2005-06-08 | 江南大学 | Coaction type food biochemical treating equipment |
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- 2015-06-25 CN CN201510355720.4A patent/CN104886715A/en active Pending
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6056884A (en) * | 1997-11-25 | 2000-05-02 | Japan As Represented By Director Of National Food Research Institute, Ministry Of Agriculture, Forestry And Fisheries | Continuous liquid pasteurizing apparatus and a method therefor |
CN1623447A (en) * | 2004-12-09 | 2005-06-08 | 江南大学 | Coaction type food biochemical treating equipment |
Non-Patent Citations (1)
Title |
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宋卫锋,等: "低压脉冲杀菌技术的试验研究", 《水处理技术》 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN116354565A (en) * | 2023-05-31 | 2023-06-30 | 曹县人民医院 | Comprehensive treatment device for hospital sewage |
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