INACTIVATION OF CONTAMINANTS USING PHOTOSENSITIZERS AND PULSED
LIGHT
CROSS-REFERENCE TO RELATED APPLICATIONS
This Application is a continuation-in-part of U.S. application No. 09/596,429 filed
June 15, 2000, and U.S application No. 60/235,999 filed September 27, 2000, which are incorporated herein in their entirety to the extent not incompatible herewith.
BACKGROUND
Contamination of blood products with infectious microorganisms such as HIN,
hepatitis and other viruses and bacteria present a serious health hazard for those who must
receive transfusions of whole blood or administration of various blood components such as
platelets, red cells, blood plasma, Factor NHL plasminogen, fibronectin, anti-thrombin in,
cryoprecipitate, human plasma protein fraction, albumin, immune serum globulin, prothrombin complex plasma growth hormones, and other components isolated from blood.
Blood screening procedures may miss pathogenic contaminants, and sterilization procedures which do not damage cellular blood components but effectively inactivate all infectious
viruses and other microorganisms have not heretofore been available.
The term "blood product" as used herein includes all blood constituents or blood
components and therapeutic protein compositions containing proteins derived from blood as
described above. Fluids containing biologically active proteins other than those derived from
blood may also be treated by the methods and devices of this invention.
The use of pathogen inactivating agents include certain photosensitizers, or compounds which absorb light of a defined wavelength and transfer the absorbed energy to an energy acceptor, have been proposed for inactivation of microorganisms found in blood
products or fluids containing blood products. Such photosensitizers may be added to the fluid
containing blood or blood products and irradiated.
The photosensitizers useful in this invention include any photosensitizers known to
the art to be useful for inactivating microorganisms. A "photosensitizer" is defined as any
compound which absorbs radiation of one or more defined wavelengths and subsequently
utilizes the absorbed energy to carry out a chemical process. Examples of such photosensitizers include porphyrins, psoralens, dyes such as neutral red, methylene blue,
acridine, toluidines, flavine (acriflavine hydrochloride) and phenothiazine derivatives, coumarins, quinolones, quinones, and anthroquinones. Photosensitizers of this invention may
include compounds which preferentially adsorb to nucleic acids, thus focusing their
photodynamic effect upon microorganisms and viruses with little or no effect upon accompanying cells or proteins. Other photosensitizers are also useful in this invention, such
as those using singlet oxygen-dependent mechanisms. Most preferred are endogenous
photosensitizers. The term "endogenous" means naturally found in a human or mammalian body, either as a result of synthesis by the body or because of ingestion as an essential foodstuff (e.g. vitamins) or formation of metabolites and/or byproducts in vivo. Examples of
such endogenous photosensitizers are alloxazines such as 7,8-dimethyl-lO-ribityl isoalloxazine (riboflavin), 7,8,10-trimethylisoalloxazine (lumifiavin), 7,8-dimethylalloxazine
(lumichrome), isoalloxazine-adenine dinucleotide (flavine adenine dinucleotide [FAD]), alloxazine mononucleotide (also known as flavine mononucleotide [FMN] and riboflavine-5-
phosphate), vitamin Ks, vitamin L, their metabolites and precursors, and napththoquinones, naphthalenes, naphthols and their derivatives having planar molecular conformations. The
term "alloxazine" includes isoalloxazines. Endogenously-based derivative photosensitizers include synthetically derived analogs and homologs of endogenous photosensitizers which may have or lack lower (1-5) alkyl or halogen substituents of the photosensitizers from which
they are derived, and which preserve the function and substantial non-toxicity thereof. When
endogenous photosensitizers are used, particularly when such photosensitizers are not
inherently toxic or do not yield toxic photoproducts after photoradiation, no removal or
purification step is required after decontamination, and a treated product can be directly
returned to a patient's body or administered to a patient in need of its therapeutic effect
without any further required processing. Using endogenous photosensitizers to inactivate
pathogens in a blood product are described in U.S. Patents No. 6,258,577 and No. 6,277,337, herein incorporated by reference.
Non-endogenous photosensitizers based on endogenous structures, such as those
described in U.S. Patent No. 6,268,120, may also be incorporated by reference herein. These non-endogenous photosensitizers and endogenously-based derivative photosentizers may be
referred to herein as endogenously-based derivative photosensitizers.
One mechanism by which these photosensitizers may inactivate pathogens is by
interfering with nucleic acids, so as to prevent replication of the nucleic acid. As used herein,
the term "inactivation of a pathogen" means totally or partially preventing the pathogen from replicating, either by killing the pathogen or otherwise interfering with its ability to
reproduce. Specificity of action of the photosensitizer is conferred by the close proximity of the photosensitizer to the nucleic acid of the pathogen and this may result from binding of the
photosensitizer to the nucleic acid. "Nucleic acid" includes ribonucleic acid (RNA) and deoxyribonucleic acid (DNA).
Microorganisms or pathogens which may be eradicated or inactivated using pathogen inactivation agents or photosensitizers include, but are not limited to, viruses (both
extracellular and intracellular), bacteria, bacteriophages, fungi, blood-transmitted parasites, and protozoa. Exemplary viruses include acquired immunodeficiency (HIN) virus, hepatitis
A, B and C viruses, sinbis virus, cytomegalovirus, vesicular stomatitis virus, herpes simplex
viruses, e.g. types I and π, human T-lymphotropic retroviruses, HTLN-m, lymphadenopathy
virus LAN/ID AN, parvovirus, transfusion-transmitted (TT) virus, Epstein-Barr virus, and
others known to the art. Bacteriophages include Φ X174, Φ 6, λ , R17, T4, and T2.
Exemplary bacteria include P. aeruginosa, S. aureus, S. epidermis, L. monocytogenes, E. coli,
K. pneumonia and S. marcescens.
The fluid to be pathogen inactivated has the photosensitizer added thereto, and then the resulting fluid mixture maybe exposed to photoradiation of the appropriate wavelength and amount to activate the photosensitizer, but less than that which would cause non-specific damage to the biological components or substantially interfere with biological activity of
other proteins present in the fluid. The wavelengths of photoradiation used may depend on
the type of photosensitizer selected and the composition of the fluid, as is known in the art.
A number of systems and methods for irradiating pathogens in a fluid with light are
known in the art. For example, U.S. Patent No. 5,762,867 is directed toward a system for
activating a photoactive agent present in a body fluid with light emitting diodes (LEDs). U.S. Patent No. 5,527,704 is directed toward an apparatus containing LEDs used to activate a fluid
containing methylene blue. U.S. Patent No. 4,880,512 uses pulsed lasers to photolyse nucleic
acids contained in a fluid. None of these above-mentioned patents uses pulsed light in combination with a photsensitizer to inactivate pathogens in a fluid containing pathogens.
The term light source or radiation source as defined herein means an emitter of radiant
energy, and may include energy in the radio, infrared, visible, ultraviolet, X-ray and/or
gainma spectra.
The photosensitizer may be added directly to the fluid to be pathogen inactivated, or may be flowed into the photopermeable container separately from the fluid being treated, or
may be added to the fluid prior to placing the fluid in the photopermeable treatment container.
The photosensitizer may also be added to the photopermeable container either before or after
sterilization of the treatment container.
The fluid containing the photosensitizer may also be flowed into and through a
photopermeable container for irradiation, using a flow through type system. Alternatively,
the fluid to be treated may be placed in a photopermeable container which is agitated and
exposed to photoradiation for a time sufficient to substantially inactivate the microorganisms, in a batch-wise type system.
The term "container" refers to a closed or open space, which maybe made of rigid or flexible material, e.g., may be a bag or box or trough. In one embodiment, the container may
be closed or open at the top and may have openings at both ends, e.g., may be a tube or tubing, to allow for flow-through of fluid therein. A cuvette has been used to exemplify one
embodiment of the invention involving a flow-through system. Collection bags, such as those
used with the Trima® and or Spectra™ apheresis systems of Gambro, Inc., (f/k/a Cobe
Laboratories, Inc., Lakewood, Colorado, USA), have been used to exemplify another
embodiment involving a batch- wise treatment of the fluid.
The term "photopermeable" means the material of the treatment container is
adequately transparent to photoradiation of the proper wavelength for activating the photosensitizer. In a flow-through system, the container has a depth (dimension measured in
the direction of the radiation from the photoradiation source) sufficient to allow
photoradiation to adequately penetrate the container to contact photosensitizer molecules at
all distances from the light source and ensure inactivation of pathogens in the fluid to be
decontaminated, and a length (dimension in the direction of fluid flow) sufficient to ensure a sufficient exposure time of the fluid to the photoradiation. The materials for making such containers, the depths and lengths of containers may be easily determined by those skilled in
the art, and together with the flow rate of fluid through the container, the intensity of the
photoradiation and the absorptivities of the fluid components, e.g., plasma, platelets, red
blood cells, will determine the amount of time the fluid should be exposed to photoradiation.
After treatment, the blood or blood product may be stored for later delivery to a
patient, concentrated, infused directly into a patient or otherwise processed for its ultimate use.
SUMMARY
The present invention provides a method and apparatus for irradiating a fluid . containing pathogens and a photoactive agent with pulsed light of an appropriate wavelength,
to activate the photoactive agent contained therein and thereby inactivate any pathogens contained in the product.
The present invention involves using a pulsed light source to generate the
photoradiation to which the fluid is exposed. The pulsing of the light may allow higher
intensities of light to be used than may otherwise be used without causing damage to either the blood product being irradiated or to the light sources due to the short duration of the radiation exposure.
Alternative sources of radiation may be used, depending on a variety of factors,
including, but not limited to the wavelength desired and the power at the desired wavelength.
LEDs (light emitting diodes) which pulse the light as it is emitted may also be used in the described invention.
The lights may be configured in many different ways, depending upon the particular apparatus being used.
One embodiment of a radiation or treatment chamber useful with the methods of the present invention includes a bank or banks or arrays of lights. Such lights may produce a
particular wavelength of light that corresponds to the particular wavelength preferred for the
type of fluid being treated, hi another embodiment, the banks or arrays of lights may be
interchangeable. For example, in one apparatus, one bank of lights that produces a
wavelength suitable for irradiating platelets may be interchanged with another bank of lights
that produces a wavelength suitable for irradiating red blood cells.
A radiation enhancer such as a second radiation source or a reflective surface may be
included in the radiation or treatment chamber. The radiation enhancer may be placed adjacent to the container containing the fluid to be irradiated or opposite the radiation source
to increase the amount of radiation contacting the fluid within the container.
The radiation or treatment chamber may also preferably include a means for producing
movement in the fluid to be irradiated. Movement provides many benefits including
improving the efficiency of the inactivation reactions by helping mix the photosensitizer with
the fluid to be photoinactivated to provide turnover of the fluid within the container at the
container-light interface.
Positioning the fluid to be inactivated so that it receives energy of sufficient
wavelength and power to induce inactivation of pathogens contained in the fluid may include a support platform, a shelf or a tray for the sample to be disposed upon; an opening or gap between two supports which may be a light or light arrays, where the fluid within the
container is positioned between the supports; or other means known in the art. The support
platform may move, as in a conveyer line, or may oscillate or agitate. The fluid-holding
shelves may be transparent to one or more of the wavelengths of light applied. The fluid
within the container may also be placed on a support surface between two or more sources of radiation, like a sandwich.
BRIEF DESCRIPTION OF THE FIGURES
Fig. 1 is a cross-sectional view of a treatment chamber which may be used in the present
invention.
Fig. 2 is a cross-sectional view of a treatment chamber like that of Fig. 1, but with an
alternative reflective surface that may also be used in the present invention.
Fig. 3 is a plan view of an array of LEDs that may be used in the present invention.
Fig. 4 is a schematic diagram of one type of LED circuit that maybe used with the present
invention.
Fig. 5a is a schematic view of the light pulses produced by a LED irradiating a container
according to the present invention.
Fig. 5b is a schematic view of the light pulses produced by an incandescent light bulb
irradiating a container according to the present invention.
Fig. 6 is another embodiment of a treatment chamber which maybe used in the present
invention.
DETAILED DESCRIPTION
Fig. 1 shows in a cross-sectional view, the inside of a radiation or treatment chamber of one type of apparatus that may be used in the present invention. It should be noted that
throughout the description of the invention, like elements have been given like numerals. The
apparatus 55, used for inactivating a fluid which may contain pathogens, consists of an
internal chamber 33 having at least one source of radiation 26. In one preferred embodiment,
the internal chamber may contain a second source of radiation 36. Each radiation source 26
and 36 respectively, is depicted as including a plurality of discrete radiation-emitting
elements. The internal chamber 33 further consists of a support platform 25 for supporting
the fluid container 10 containing the fluid to be irradiated, and a control unit 11.
As introduced above, two sources of radiation are shown within internal chamber 33.
Radiation source 26 may be located along the top portion of the internal chamber 33 above
the container 10 which holds or contains the fluid to be irradiated, while radiation source 36
maybe located along the bottom portion of the internal chamber 33 below the container 10.
Although not shown, radiation sources may also be located along some or all of the sides of
the internal chamber 33 perpendicular to the container 10. The radiation or treatment
chamber 55 may alternatively contain a single radiation source at any location within the
internal chamber 33 and still comply with the spirit and scope of the present invention.
As shown in Fig. 1, the radiation source including a plurality of radiation-emitting
elements collectively designated as source 26 includes an upper support substrate 15
containing a plurality of discrete radiation emitting elements or discrete light sources (see
discrete source 20 as one example) mounted thereon. The support substrate 15 maybe in an
arcuate shape as shown, in a flat shape, or in other configurations which are not shown. Thus,
the upper support substrate 15 could also be in a shape other than arcuate without departing from the spirit and scope of the invention.
As further depicted in Fig. 1, the radiation source collectively designated as discrete
source 36 includes a lower support substrate 35 which also contains a plurality of discrete
radiation emitting elements or discrete light sources (see discrete source 30 as another
example). Lower support substrate 35 preferably runs parallel to support platform 25. The
lower support substrate 35 may be substantially flat as shown, or may be in an arcuate shape
similar to element 15 above, or maybe in a shape other than arcuate, without departing from
the spirit and scope of the invention.
The support substrates 15 and 35 may include at least one reflective surface, and as
shown, may include two or more reflective surfaces 19 and 37 thereon. Reflective surface 17
is shown as running contiguous with upper support substrate 15. Reflective surface 37 is
shown as running contiguous with lower support substrate 35. The reflective surfaces 17 and
37 may also run contiguously with only a portion of support substrates 15 and 35. As shown
in Fig. 1, discrete light source devices 20 and 30 extend outwardly away from the surface of
the support substrates 15 and 35. Alternatively, a discrete light source could be recessed into
the surface such that the surface surrounds each discrete light source in a parabolic shape (not
shown). The support substrate may or may not have reflective surfaces. Most preferably, the reflective surface may not contain any light sources. Such a reflective surface containing no
light sources (not shown) may be located within the treatment chamber on a side opposite
from the radiation source. As shown in Fig. 2, the support platform 25 may have a reflective
surface 39. This reflective surface 39 on support platform 25 may be in place of, or may be in
addition to another reflective surface (see element 17 as one example) within the treatment
chamber. There may also be no reflective surfaces at all within the treatment chamber.
In any of these reflective surface embodiments, the reflective surface maybe coated
with a highly reflective material which serves to reflect the radiation pulses emitted from the
lights back and forth throughout the treatment chamber until the pulse is preferably completely absorbed by the fluid being irradiated. The highly reflective nature of the
reflective surface reflects the emitted light back at the fluid-filled bag or container 10 with minimum reduction in the light intensity.
As shown in Fig. 1, support platform 25 is positioned within the internal treatment
chamber 3. The support platform 25 may be located substantially in the center of the
radiation or treatment chamber (as shown in Fig. 1), or may be located closer to either the top
portion or the bottom portion of the treatment chamber without departing from the spirit and
scope of the present invention. The support platform 25 supports the container 10 containing
the fluid to be irradiated. The support platform 25 may also be defined as a tray or a shelf.
Additionally or alternatively, the platform 25 may be made of a photopermeable material to
enable radiation emitted by the pulsed lights to be transmitted through the platform and
penetrate the fluid contained within the container 10. The platform may also be a wire or
other similar mesh-like material to allow maximum light transmissivity therethrough. The
support platform 25 is preferably capable of movement in multiple directions within the
treatment chamber. An agitator, such as a Helmer flatbed agitation system available from
Helmer Corp. (Noblesville, IN, USA) may also be used. This type of agitator provides
oscillary motion. Other types of agitators may also be used to provide motion to the fluid
contained within the container 10, without departing from the spirit and scope of the
invention. The support platform 25 may alternatively rotate in multiple possible directions
within the radiation chamber in varying degrees from between 0° to 360°. The support
platform 25 may also oscillate back and forth, or side to side along the same plane. As a
further alternative, one or more of the light sources may also move in a coordinated manner
with the movement of the support platform. Such oscillation or rotation would enable the
majority of the photosensitizer and fluid contained within the container 10 to be exposed to
the light emitted from each of the discrete radiation sources (e.g. discrete sources 20 and 30),
by continually replacing the exposed fluid at the light-fluid interface with fluid from other parts of the bag not yet exposed to the light pulses. Such mixing brings to the surface new
fluid to be exposed to light with each new pulse of radiation.
The movement of both the support platform 25 and/or the radiation sources 26 and 36
may be controlled by control unit 11. The control unit 11 may control the rate of light
emission in either a continuous or a pulsed mode, and the control unit 11 may be provided
with a selector switch (not shown) in order to select which mode is to be employed. In the
"continuous" mode the radiation source emits radiation in a continuous manner, hi the "pulsed" mode the radiation source emits pulses of radiation.
As shown in Fig. 3, each radiation source 26 may consist of a bank or array of a
plurality of discrete LEDs devices. LED devices 20, 21 and 22 are self-contained emitters of
radiation. Each LED emits a single color of light when an electrical current is applied.
Each of the LED devices in the array 26 may emit the same wavelengths of light, or
because each source is discrete, each source may emit particular wavelengths of radiation
independently of the emission of radiation by any other light source devices also contained
within the light array. For example, LED device 20 may emit light of different wavelengths
than the wavelengths emitted by LED device 22. This could be useful in providing different
wavelengths of light for different blood products, as may be desired.
The discrete radiation sources or lights may be arranged in banks or arrays containing
multiple rows of individual lights, or may be arranged in a single row (not shown). As shown
in Fig. 3, if LED devices are used, a plurality of discrete LED devices may be arranged in
multiple rows. The lights may also be staggered or offset from each other (not shown). If a
bank or an array of LED lights is located in both the top and the bottom of the irradiation
chamber 55 (see Fig. 1), each bag or container 10 containing fluid to be irradiated will be
exposed to light pulses on both the top and the bottom surfaces of the bag 10. A reflective
surface 17 (like that shown in Fig. 1) may also be part of the array.
As described, the lights used in this invention may be LED devices. LED devices are
available from any one of a number of companies. Some companies that manufacture LED
devices useful in this invention are Cree, Inc. (Durham, NC, USA); Nichia, Co. (Tokushima, JP); Kingbright, Corp. (City of Industry, CA, USA) and Lumileds Lighting, LLC (San Jose,
CA, USA).
The light devices 20, 21, and 22 maybe placed in an interchangeable format which would allow one set of LED devices to be removed and replaced by another set of LED
devices. For example, depending upon the type of blood product to be irradiated, the LED
devices which emit particular wavelengths of light that correspond to the type of blood
product being irradiated may be easily inserted into the irradiation chamber. As one example,
one array containing a radiation source which corresponds to a first type of blood product is
inserted into the treatment chamber. After irradiation of the first blood product, the array may
be removed from the treatment chamber. If a second type of blood product needs to be
treated, a second array corresponding to the second type of blood product is inserted into the
treatment chamber. After irradiation of the second blood product the second array may be removed from the treatment chamber.
Alternatively, as introduced above, a single bank of LED devices may contain
multiple discrete light sources which each emit a variety of different wavelengths for this
purpose as well. Control unit 11 (see Fig. 1) maybe used to selectively engage the particular
discrete LED sources which emit light pulses of particular wavelengths, depending upon the
type fluid to be irradiated. These concepts may be used with non-LED light devices as well.
The radiation sources used in this invention are most preferably capable of being
pulsed, hi some embodiments, pulsed radiation sources may be generated by intense flashes
from an inert gas such as xenon and each pulse can last less than one millisecond in duration.
In an alternative system using LEDs capable of being pulsed, one method of
generating light pulses may be by a series of switches such as those shown schematically in Fig. 4. Fig. 4 shows one of many possible schematic LED circuits which may be used in this
invention. Each discrete radiation source (see LED devices 20, 21 and 22 as examples) is
arranged in the form of a LED matrix 3 comprising a plurality of parallel branches (see
branch 24 as one example). Each branch, such as branch 24 contains a predetermined number
of discrete LED devices (see devices 20, 21 and 22) preferably in series. For example, LED
devices 20, 21 and 22 may be connected to one another in series and together constitute one
parallel branch 24 of the diode matrix 3. The current flowing through each branch may be
limited by means of a series resistor (element 5 for one such example). Each branch may be
connected to one of a plurality of switches (see switches 7a-g) which are capable of being
opened and closed. When switch 7a (as one such example) is in a closed position (not
shown) current is able to flow from the voltage source 8, through switch 7a, through the
resistor 5, to each discrete LED 20, 21 and 22 located within branch 24. When switch 7a is
open as shown in Fig. 4, no current is able to flow down that particular branch to light each
discrete LED contained within this branch. The control unit (see element 11 in Fig. 1) may
be connected to any one or more of switches 7a-g. Each switch may thereby be controlled
individually, or in coordination with one or more of the other switches in the matrix 3 to provide current to the desired branch or branches. Determination of the switching sequence
may depend on the type and/or duration of the pulse wanted, which in turn may be determined
by the type of fluid being irradiated. In another embodiment (not shown) a varistor may be
used in place of any one or more of the resistors (see element 5 as an example). The varistor may alternatively be used to enable the LEDs to be pulsed on or off, as well as to brighten
and/or dim the light intensity. Any one of a number of commonly used switching
mechanisms known in the art may be used in providing the desired pulsing of this invention.
Figs. 5a and 5b show alternative cross-sectional views of light pulses possibly
produced by different types of light sources. Fig. 5a shows a LED 20 emitting light pulses 71
which irradiate bag 10. The light pulses could be in the visible or the UN spectrum or both,
depending upon the type of fluid being irradiated in bag 10. Fig. 5b shows an incandescent
light source 23 which may also emit pulses of light 81 in the desired wavelength for the
particular product being irradiated.
Fig. 6 shows an alternative embodiment of an irradiation or treatment chamber to be
used with the present invention. A bank of pulsed light sources 50 may be located within the
top of the irradiation chamber extending from lid 40. A reflective surface 57 is shown as part
of the inner surface of lid 40, however, reflective surface 57 or another one or more surfaces
(not shown) may be located anywhere within the radiation chamber as introduced above.
The lid 40 is capable of being opened and closed. During exposure of the bag 10
containing the fluid to be irradiated to the light sources, the lid 40 is in a closed position (not
shown). To add or remove the bag 10 containing the fluid to be irradiated from the
irradiation chamber, a drawer 45 located on the front of the irradiation chamber may be
disposed in an open position (as shown). During the irradiation procedure, the drawer 45 is
placed in a closed position (not shown).
The pulsed light sources 50 may be fluorescent or incandescent tubes, which stretch the length of the irradiation chamber, or maybe a single light source which extends the length
and width of the entire chamber (not shown). The LEDs shown in Fig. 3 may also be used in
this embodiment. Although lights are shown as being located only on the top of the
irradiation chamber, lights may also be located in the bottom of the chamber as well. Some
examples of light sources that may be used include those available from the Xenon
Corporation (Woburn, MA, USA) or from PurePulse Technologies, Inc. (San Diego, CA,
USA) among other companies.
As shown in Fig. 6, the support platform 67 may be located within and/or forming part
of drawer 45. The support platform 67 may contain gaps 60 or holes or spaces within the
platform 67 to allow radiation to penetrate through the gaps directly into the container 10
containing fluid to be irradiated.
Although pulsed light systems may generally maintain the system (light sources and
container) at cooler temperatures, a cooling system may also optionally be included. As
shown in Fig. 6, air cooling using at least one fan 65 may be preferred but it is understood that other well-known systems can also be used. Although not shown in Fig. 6, the method may also include the use of temperature sensors and other cooling mechanisms where
necessary to keep the temperature below temperatures at which desired proteins and blood
components in the fluid being irradiated are damaged. Preferably, the temperature is kept
between about 0° C and about 45° C, more preferably between about 4° C and about 37° C,
and most preferably about 28° C.
The pulsed light used may be in the visible spectrum, the ultraviolet spectrum, or both
the visible and ultraviolet spectra. Any suitable wavelength or wavelengths of light may be
used in any proportion and amount of energy that produces the desired level of inactivation of pathogens in the fluid being irradiated. As one example, half the light emitted may be in the visible spectrum and about half the light emitted may be in the ultraviolet spectrum. As
another alternative, not meant to be limiting, about one-third of light maybe in one spectral
range and the other two-thirds of light in a different spectral range.
In one embodiment of the invention, multiple light sources (or multiple arrays of light sources) may be used to provide multiple wavelengths of light.
The pulsed light sources may also be selected so that each discrete radiation source or
device has either a broad band or narrow band spectrum. Broad band pulsed light sources
include light in both the visible and ultraviolet range and/or in the infrared wavelength range. Thus, a single light source can provide a mixture of both visible and ultraviolet light. If
ultraviolet light is used, the amount of ultraviolet light is preferably kept to a level that minimizes non-specific damage to desired fluid components. Generally, this may be provided
by using 50% or less ultraviolet light relative to the total light energy delivered. Non-specific
damage is damage that damages components contained within the fluid being irradiated. A
narrow band pulsed light source is preferably used with respect to irradiation of the selected blood components.
The photoradiation in both the ultraviolet and visible spectra may be supplied concurrently or sequentially, with the visible portion preferably being supplied first. As
described above, the photoradiation source may be a simple lamp or may consist of multiple
lamps radiating at differing wavelengths. The photoradiation sources may also consist of
multiple discrete LED devices radiating at the same or different wavelengths. Individual
LED devices which emit different wavelengths of light and pulse in differing frequencies may
be combined together in one array. Alternatively, one array could contain multiple LED
devices which all emit light in the same wavelength range.
The particular parameters of each pulsed light source can be selected to provide irradiation of sufficient intensity to activate the photosensitizer in a pathogen inactivation
process without damage to the blood or blood components being irradiated. The
photoradiation source should be capable of delivering a sufficient amount of light to activate the photosensitizer, preferably from about 3 to at least about 200 J/cm2. The preferred range
of wavelengths of light supplied in pulsed light fashion in accordance with the present
invention are between 280-550 nm. However, the preferred wavelengths of light used may
depend upon the type of photosensitizer used. LEDs or other types of lights which emit
pulses of light having wavelengths of around 400-550 nm may be used for irradiating
platelets, LEDs or other types of lights which emit pulses of light emitting wavelengths of around 260-500 nm maybe used for irradiating plasma, and LEDs or other types of lights
emitting pulses of light having wavelengths of around 430-550 nm may be used for irradiating RBCs.
The pulsed light sources may emit pulses of radiation at pulse rates of between one
pulse per 0.25 seconds up to one pulse per 100 seconds. Each individual pulse may last
between 0.1 and 10 seconds.
The pulsed light sources produce their high-intensity, short duration output pulses in a
repetitive fashion. To insure that all of the fluid to be irradiated is subjected to substantially
the same irradiation conditions, each discrete light device should produce the same amount of
repetitions, and the bag should be placed in a location which exposes the greatest possible
surface of the bag to radiation.
Pulsed irradiation using very high energy, short duration pulses may have advantages
over lower energy continuous irradiation. The pulsing of the light source may allow for a
higher intensity than could otherwise be used without damage to either the blood product or
to the light sources. The high energy pulses may also penetrate deeper into the fluid being
irradiated. This would likely enhance pathogen inactivation, especially in more opaque blood
products such as red blood cells or plasma containing high levels of lipids. In more opaque solutions, mixing is enhanced because the thicker layers of solution photolyzed by the high intensity flash are turned over more readily. With low intensity light, thinner layers of
photolyzed solution must be turned over, and this is more difficult to achieve in a system
being mixed.
Pulsing the light instead of providing continuous light emission is beneficial for
several reasons. The intensity of light which may be emitted from the radiation sources may be increased dramatically if the lights are allowed to be turned off and rested in between light
pulses. As stated above, pulsing the light at a high intensity also allows for greater depth of light penetration into the fluid being irradiated, thus irradiating a thicker layer of fluid with
each pulse.
Synchronizing the radiation sources to pulse in a coordinated manner with the
movement of the support platform allows for fluid which has already been irradiated to be
replaced by fluid which has not been irradiated. One such sequence may be as follows. The
radiation sources are pulsed on for a period of time, perhaps for between one and ten seconds.
The radiation sources_are then pulsed off for another period of time, perhaps again for between one and ten seconds. While the radiation source is off, the support platform moves in a manner as described above, to replace the fluid which had been exposed to radiation with
the previous pulse, with fluid which has not yet been exposed to radiation. When the
radiation sources are then pulsed on again, new, unexposed fluid is now exposed to the
radiation sources.
Synchronizing pulses to mixing cycles may also optimize the inactivation process. If
more than one bank or array of pulsing lights are used, each bank or array may be
programmed to pulse simultaneously with the other banks of lights, or may be programmed to
pulse the lights independently of the other banks of lights.
Pulsed light sources may also allow for adjustment of the duration and intensity of
light energy per pulse to assure optimum eradication of the viruses or pathogens with the least amount of exposure of the blood product to light. The lights may be pulsed in a frequency
corresponding to the rocking of the support platform, as set forth above. Alternatively, the
lights may not pulse completely on and then pulse completely off. The lights may pulse in an
increasing or decreasing frequency of intensity. For example, the lights could dim in
brightness and then could increase in brightness or intensity. The lights may also pulse in
differing cycles or frequencies depending on the type of fluid being inactivated.
It should be understood that various changes and modifications to the presently
preferred embodiments described herein will be apparent to those skilled in the art. Such changes and modifications can be made without departing from the spirit and scope of the
present invention and without diminishing its attendant advantages.